The　recently　proposed　DEnitrifying　AMmonium　OXidation　(DEAMOX)　process　combined　anaerobic　ammonia　oxidation　(ANAMMOX)　with　denitrification　to　convert　nitrate　to　nitrite,　which　was　a　promising　way　for　treating　wastewater　containing　nitrate　and　ammonia.　This　study　investigated　the　feasibility　of　establishing　DEAMOX　process　by　seeding　partial　denitrification　sludge　(NO3-　-＞　NO2-)　using　sodium　acetate　as　an　electron　donor　in　a　sequencing　batch　reactor.　Results　showed　that　the　DEAMOX　process　was　established　successfully　and　operated　stably　in　114-days　operation.　The　average　effluent　total　nitrogen　concentration　was　below　5　mg　L-1　and　TN　removal　efficiency　reached　up　to　97%　at　COD/NO3-　ratio　of　3.0　under　initial　NH4+　concentration　of　25　mg　L-1　and　NO3-　of　30　mg　L-1.　It　suggested　that　the　presence　of　NO2-　in　the　system　supplied　for　ANAMMOX　and　the　relatively　long　sludge　retention　time　(SRT)　for　denitrifiers　were　attributed　to　commendable　coexistence　of　ANAMMOX　and　denitrifying　bacteria.　(C)　2015　Elsevier　Ltd.　All　rights　reserved.