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Abstract:
In this paper, we report the improvement of PCR microfluidic chip efficiency achieved by coating the inner surface of steel capillary microchannel with a 22-A mu m film of the ultraviolet-solidified NOA61 using a device invented by us. Our results indicate that with this treatment, the roughness of the inside wall of steel capillary was improved from Ra = 0.921 to Ra = 0.254. The contact angle was decreased from about 95A degrees to 56A degrees, and the surface hydrophobicity was also increased. The flow pressure for performing the real-time PCR in the microfluidic chip with modified surface was reduced by twofold (2.11/1) and that resulted in a substantially increased efficiency of PCR. A modification of the microchannel interior surface improved the quality of the on-chip integrated PCR procedure.
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CELL BIOCHEMISTRY AND BIOPHYSICS
ISSN: 1085-9195
Year: 2015
Issue: 2
Volume: 72
Page: 605-610
2 . 6 0 0
JCR@2022
ESI Discipline: BIOLOGY & BIOCHEMISTRY;
ESI HC Threshold:254
JCR Journal Grade:3
CAS Journal Grade:4
Cited Count:
WoS CC Cited Count: 6
SCOPUS Cited Count: 6
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 2
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