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Author:

Wu, Jian (Wu, Jian.) | Guo, Wei (Guo, Wei.) | Wang, Chunyan (Wang, Chunyan.) | Yu, Kuanxin (Yu, Kuanxin.) | Ma, Ying (Ma, Ying.) | Chen, Tao (Chen, Tao.) | Li, Yinghui (Li, Yinghui.)

Indexed by:

Scopus SCIE PubMed

Abstract:

In this paper, we report the improvement of PCR microfluidic chip efficiency achieved by coating the inner surface of steel capillary microchannel with a 22-A mu m film of the ultraviolet-solidified NOA61 using a device invented by us. Our results indicate that with this treatment, the roughness of the inside wall of steel capillary was improved from Ra = 0.921 to Ra = 0.254. The contact angle was decreased from about 95A degrees to 56A degrees, and the surface hydrophobicity was also increased. The flow pressure for performing the real-time PCR in the microfluidic chip with modified surface was reduced by twofold (2.11/1) and that resulted in a substantially increased efficiency of PCR. A modification of the microchannel interior surface improved the quality of the on-chip integrated PCR procedure.

Keyword:

NOA 61 Surface modification Microfluidic chip

Author Community:

  • [ 1 ] [Wu, Jian]Beijing Univ Technol, Inst Laser Engn, Beijing 100124, Peoples R China
  • [ 2 ] [Guo, Wei]Beijing Univ Technol, Inst Laser Engn, Beijing 100124, Peoples R China
  • [ 3 ] [Ma, Ying]Beijing Univ Technol, Inst Laser Engn, Beijing 100124, Peoples R China
  • [ 4 ] [Chen, Tao]Beijing Univ Technol, Inst Laser Engn, Beijing 100124, Peoples R China
  • [ 5 ] [Wang, Chunyan]Chinese Astronaut Sci Res & Training Ctr, State Key Lab Space Med Fundamentals & Applicat, Beijing 100094, Peoples R China
  • [ 6 ] [Li, Yinghui]Chinese Astronaut Sci Res & Training Ctr, State Key Lab Space Med Fundamentals & Applicat, Beijing 100094, Peoples R China
  • [ 7 ] [Yu, Kuanxin]Beijing Univ Technol, Coll Appl Sci, Beijing 100124, Peoples R China

Reprint Author's Address:

  • [Wu, Jian]Beijing Univ Technol, Inst Laser Engn, Beijing 100124, Peoples R China

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Source :

CELL BIOCHEMISTRY AND BIOPHYSICS

ISSN: 1085-9195

Year: 2015

Issue: 2

Volume: 72

Page: 605-610

2 . 6 0 0

JCR@2022

ESI Discipline: BIOLOGY & BIOCHEMISTRY;

ESI HC Threshold:254

JCR Journal Grade:3

CAS Journal Grade:4

Cited Count:

WoS CC Cited Count: 6

SCOPUS Cited Count: 6

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

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