A　bacterial　strain　(designated　as　N4)　of　Pseudoxanthomonas　was　isolated　from　a　denitrification　biofilter　reactor.　This　study　examined　its　degradation　capability　of　di(2-ethylhexyl)　phthalate　(DEHP)　and　its　denitrification　ability.　All　results　showed　that,　strain　N4　could　use　DEHP　as　a　carbon　and　energy　source.　Strain　N4　demonstrated　good　DEHP　degradation　ability　over　a　broad　pH　and　temperature　range.　The　optimal　temperature　and　pH　for　DEHP　degradation　were　32　degrees　C　and　6.0,　respectively.　The　kinetics　of　DEHP　degradation　by　strain　N4　were　exponential　in　nature.　Strain　N4　showed　denitrification　ability　and　could　reduce　nitrate　and　nitrite　but　not　with　DEHP　as　its　carbon　source.　(C)　2014　Elsevier　Ltd.　All　rights　reserved.