During　the　past　decades,　layer-by-layer　(LbL)　assembly　technique　based　on　electrostatic　adsorption　of　oppositely　charged　polyelectrolytes　(PEs)　has　been　one　of　the　most　frequently　utilized　processes　for　assembling　ultra-thin　film.　However,　LbL　process　is　also　recognized　as　a　time-consuming　process,　especially　for　assembly　of　dense　membranes.　In　this　paper,　a　one-step　dynamic　assembly　based　on　electrostatic　adsorption　was　developed　to　prepare　the　PE　complex　membranes.　The　chitosan　(CS),　used　as　a　model　polycation,　was　dynamically　deposited　on　a　hydrolyzed　polyacrylonitrile　(PAN)　support　membrane　with　negative　charges　under　a　selected　pressure　to　form　a　PE　complex　membrane　for　pervaporation　separation　of　water-ethanol　mixture.　Compared　with　traditional　dip-coating　method,　this　new　assembly　process　could　offer　some　advantages　such　as　the　formation　of　selective　layer　with　dilute　solution,　easily　used　for　different　membrane　module　types　and　suitable　for　large　scale　applications.　The　assembly　conditions　such　as　dynamic　pressure,　CS　molecular　weight,　and　CS　concentration　were　investigated.　The　appropriate　conditions　for　assembly　were:　dynamic　pressure.　0.2　MPa;　CS　molecular　weight,　82,000;　and　CS　concentration,　0.5　wt.%.　In　the　case　of　pervaporation　of　95　wt.%　ethanol/water　mixtures,　the　separation　factor　and　the　permeate　flux　of　the　CS　polyelectrolyte　complex　membranes　were　422　and　230　g/(m(2)　h)　(60　degrees　C),　respectively.　The　cross-linking　treatment　with　glutaraldehyde　and　sulfuric　acid　were　subsequently　performed.　It　was　found　that　the　separation　performance　was　improved　by　the　cross-linking　treatment.　Finally,　inner　skin　hollow　fiber　PE　complex　membranes　were　also　successfully　explored　using　one-step　dynamic　assembly　for　pervaporation　dehydration.　The　proposed　process　will　offer　a　new　approach　to　obtain　a　relatively　integrated　PE　complex　membrane.　(C)　2009　Elsevier　B.V.　All　rights　reserved.