Nitrogen　removal　via　the　nitrite　pathway　has　the　potential　of　reducing　the　requirements　for　aeration　consumption　and　carbon　source.　However,　the　development　of　an　efficient　and　quick　start-up　strategy　for　partial　nitrification　to　nitrite　has　proven　difficult　in　the　treatment　of　low　strength　wastewater.　In　this　study,　the　feasibility　of　partial　nitrification　achieved　by　using　real-time　aeration　duration　control　was　not　only　demonstrated　from　the　kinetic　mechanism,　but　also　was　validated　in　three　sequencing　batch　reactors　(SBRs)　fed　with　low　C/N　domestic　wastewater.　Nitrite　accumulation　could　be　achieved　when　aeration　was　terminated　as　soon　as　an　inflexion　pH　point　was　reached　(the　dpH/dt　became　from　negative　to　positive).　The　reduction　or　limitation　of　the　NOB　growth　could　be　achieved　through　aeration　duration　control,　due　to　leaving　no　extra　time　for　NOB　to　convert　the　accumulated　nitrite.　The　experimental　operation　results　also　showed　that　partial　nitrification　with　nitrite　accumulation　ratios　of　over　80%　was　achieved　successfully　in　these　three　reactors　with　process　control.　Fluorescence　in　situ　hybridization　(FISH)　analysis　indicated　the　reduction　of　NOB　was　achieved　and　AOB　became　the　dominant　nitrifying　bacteria.　Moreover,　an　integrated　start-up　strategy　based　on　aeration　duration　control　was　proposed　to　quickly　achieve　partial　nitrification　to　nitrite.