This　study　investigated　the　effects　of　five　cephalosporin　antibiotics　(ceftazidime,　ceftriaxone,　cefdinir,　cefixime　and　cefepime)　on　performance　and　bacterial　community　structure　in　bio-electrochemical　systems　(BES)　and　sequencing　batch　biofilm　reactor　(SBBR).　The　results　showed　that　the　external　electric　field　had　no　significant　effect　on　the　removal　of　COD　and　ammonia　nitrogen　in　water.　The　removal　rates　of　five　antibiotics　in　BES　increased　by　28.5%,　20.0%,　9.1%,　21.0%,　and　11.5%,　respectively.　High-through　sequencing　showed　that　microbial　membrane-growing　process　increased　species　diversity,　and　antibiotics　had　a　significant　inhibitory　effect　on　the　initial　biofilm　of　the　reactor.　As　time　progressed,　the　inhibitory　effect　was　weakened,　and　the　microorganism　were　tolerated　and　re-enriched.　The　increase　in　the　type　and　concentration　of　antibiotics　and　the　applied　electric　field　had　a　significant　effect　on　the　microorganisms　in　the　reactor.　The　dominant　microorganisms　for　antibiotic　removal　in　the　SBBR　wereLuteococcus,Cloacibacterium,Dysgonomonas,　andOttowia.　The　dominant　bacteria　in　the　BES　wereOttowiaandTahibacte.　The　abundance　of　these　strains　increased　significantly　during　antibiotic　acclimation.　The　abundance　ofOttowia,Tahibacter,　andNakamurellawere　significantly　higher　than　SBBR.　Thus　the　BES　system　had　a　good　antibiotic　degradation　effect.　The　BES　can　effectively　treat　simulated　domestic　sewage　containing　multiple　antibiotics,　laying　a　theoretical　foundation　for　the　actual　wastewater　treatment.