Fluorescence　microscopy　is　widely　used　in　various　of　practical　applications　now.　High　resolution　optical　sectional　microscopic　imaging　utilized　by　confocal　two-or　multi-photon　fluorescence　microscopy　has　became　an　essential　tool　in　biological　researches.　However,　optical　aberrations　introduced　by　nonhomogeneity　refractive　index　of　tissues　degraded　the　resolution　and　brightness　of　the　images.　Here　we　present　the　implementation　of　self-interference　digital　holographic　adaptive　optics　in　fluorescence　microscopy.　Wavefront　sensing　and　correction　is　achieved　by　holographic　recording　and　numerical　processing　approach,　dispenses　with　Shack-Hartmann　sensor　and　deformable　mirror-based　complicated　system.　The　operation　speed　of　the　system　is　enhanced　using　off-axis　Fourier　triangular　holography.　Both　the　influence　of　the　size　and　axial　position　of　the　guide　star　on　the　quality　of　the　corrected　images　are　investigated.