This　paper　describes　a　method　for　detecting　small　ventricular　volume　differences　between　experimental　group　and　non-drug　using　control　group　in　mouse　brain.　Deformations　are　obtained　by　a　three　step　intensity-based　nonlinear　registration　routine　that　transforms　one　brain　to　another.　High　resolution　T2-weighted　mouse　brain　magnetic　resonance　microscopy　(MRM)　was　analyzed　using　full　deformation　tensor.　A　significant　mean　Jacobian　determinant　increase　was　found　in　the　experimental　group　(0.151+/-0.029,　p＜0.001,　two-tailed　t-test)　but　not　in　the　control　group　(0.021+/-0.027,　p=0.07).　Moreover,　the　increase　in　mean　ventricular　Jacobian　determinant　in　the　experimental　group　is　significantly　greater　than　in　the　control　group　(p＜0.001).　This　approach　may　be　used　to　investigate　the　increasing　in　ventricular　volume　in　transgenic　mice　and　other　putative　animal　models　of　Alzheimer＇s　disease.