3D Imaging and Quantification of the Integrin at a Single-Cell Base on a Multisignal Nanoprobe and Synchrotron Radiation Soft X-ray Tomography Microscopy. - Details

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The　development　of　three-dimensional　(3D)　single-cell　imaging　and　protein　quantitative　methods　can　provide　more　comprehensive　information　for　diagnoses.　We　report　the　design　and　synthesis　of　a　multisignal　nanoprobe　(AuGdNC@BSA-CV)　for　single-cell　3D　imaging　and　quantifying　the　integrin　αIIbβ3　using　correlated　synchrotron　radiation　soft　X-ray　tomography　microscopy　and　an　iterative　tomographic　algorithm　termed　equally　sloped　tomography　for　the　first　time.　Moreover,　on　the　basis　of　the　Au　or　Gd　content　of　our　nanoprobe,　the　number　of　integrin　αIIbβ3　on　a　single　cell　also　can　be　accurately　quantified　(1.5　×　107　per　cell)　via　inductively　coupled　plasma　mass　spectrometry.

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[ 1 ] [Zhang Chunyu]CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Beijing 100090, China
[ 2 ] [Yao Shengkun]School of Physical Science and Technology, Shanghai Tech University, Shanghai 201210, China
[ 3 ] [Xu Chao]College of Chemistry and Material Science, Shandong Agricultural University, Tai'an 271018, China
[ 4 ] [Chang Yanan]CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
[ 5 ] [Zong Yunbing]School of Physical Science and Technology, Shanghai Tech University, Shanghai 201210, China
[ 6 ] [Zhang Kai]Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
[ 7 ] [Zhang Xiangzhi]Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
[ 8 ] [Zhang Lijuan]Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
[ 9 ] [Chen Chunying]CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Beijing 100090, China
[ 10 ] [Zhao Yuliang]CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Beijing 100090, China
[ 11 ] [Jiang Huaidong]School of Physical Science and Technology, Shanghai Tech University, Shanghai 201210, China
[ 12 ] [Gao Xueyun]Department of Chemistry and Chemical Engineering, Beijing University of Technology, Beijing 100124, China
[ 13 ] [Wang Yaling]CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Beijing 100090, China

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Analytical chemistry

ISSN： 1520-6882

Year： 2021

Issue： 3

Volume： 93

Page： 1237-1241

7 . 4 0 0

JCR@2022

ESI HC Threshold：96

JCR Journal Grade：1

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count： 22

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 8

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化学与生命科学学院本学院/部未明确归属的数据

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