The　development　of　three-dimensional　(3D)　single-cell　imaging　and　protein　quantitative　methods　can　provide　more　comprehensive　information　for　diagnoses.　We　report　the　design　and　synthesis　of　a　multisignal　nanoprobe　(AuGdNC@BSA-CV)　for　single-cell　3D　imaging　and　quantifying　the　integrin　alpha(IIb)beta(3)　using　correlated　synchrotron　radiation　soft　X-ray　tomography　microscopy　and　an　iterative　tomographic　algorithm　termed　equally　sloped　tomography　for　the　first　time.　Moreover,　on　the　basis　of　the　Au　or　Gd　content　of　our　nanoprobe,　the　number　of　integrin　alpha(IIb)beta(3)　on　a　single　cell　also　can　be　accurately　quantified　(1.5　x　10(7)　per　cell)　via　inductively　coupled　plasma　mass　spectrometry.