HIV-1　integrase　(　IN)　is　an　essential　enzyme　for　HIV-1　replication,　so　it　can　also　be　regarded　as　an　attractive　target　for　finding　new　drugs,　but　still　no　drug　of　anti　-　IN　come　into　market.　Up　to　now,　most　of　anti　-　HIV　drugs　can　make　the　virus　drug　resistant.　New　drug　discovery　is　important　to　Highly　Active　Anti　-　Retroviral　Therapy　(HAART).　Pharmacophore　is　a　powerful　tool　for　new　drug　discovery　and　design.　At　present,　there　are　some　studies　on　DKA　Pharmacophores,　but　all　have　shortcomings.　This　study　derived　Pharmacophore　based　on　five　IN　-　DKA　complexes,　not　only　used　X　-　ray　structure.　Then　the　mode　was　refined　by　an　effective　binding　model　generated　by　comparing　drug　resistance　mutant　complexes　with　wild　-　type　IN　complex.　It　made　the　pharmacophore　more　reasonable　and　effective.　The　refined　model　can　be　used　to　identify　novel　inhibitors.