To　probe　into　the　feasibility　of　screening　anti-HIV　compounds　by　using　HIV-1　p24　detection　kit　made　by　Hebei　Medical　University.The　sensitivity,　reproducibility　and　efficacy　of　the　Hebei　p24　kit　were　evaluated　compared　with　the　commercially　available　Vironostika　HIV-1　Antigen　Microelisa　System　(Biomerieux).Hebei　p24　kit　had　high　sensitivity　and　good　reproducibility.　In　vitro　screening　demonstrated　that　there　was　no　statistically　significant　difference　(P　greater　than　0.05)　between　these　two　kits　in　assessing　anti-HIV　compounds.Hebei　p24　kit　could　be　used　as　an　easily　affordable　alternative　method　for　detection　of　HIV-1　in　screening　anti-HIV　compounds.