Steroid　estrogens,　as　typical　endocrine　disrupting　chemicals　(EDCs),　have　raised　an　increasing　concern　due　to　their　endocrine　disrupting　effects　on　aquatic　animals　and　potential　hazards　on　human　health.　Batch　experiments　were　conducted　to　study　17　beta-estradiol　(E2)　removal　and　Estradiol　Equivalent　Quantity　(EEQ)　elimination　by　anaerobic　granular　sludge　(AnGS)　combined　with　different　valence　iron　sources.　Results　showed　that　E2　was　effectively　biodegraded　and　transformed　into　E1　by　AnGS.　The　addition　of　different　valence　iron　sources　all　promoted　E2　degradation,　reduced　E2　Equivalent　Quotient　(EEQ)　concentration,　and　increased　methane　production　in　the　batch　experiments.　The　enhancement　effect　of　zero-valent　iron　(ZVI)　on　E2　removal　and　EEQ　elimination　was　stronger　than　that　of　Fe2+　and　Fe3+　in　our　experiments.　The　enhancement　effect　proportion　of　ZVI　corrosion,　Fe2+,　and　Fe3+　in　the　process　of　E2　degradation　by　AnGS　combined　with　ZVI　were　42.26%,　40.21%　and　17.53%,　respectively.