A　continuous　flow　reactor　(CFR)　and　a　sequencing　batch　reactor　(SBR)　were　operated　in　parallel　to　investigate　the　difference　between　anammox　granulation　in　CFR　and　SBR　under　decreasing　temperature　and　no　strict　anaerobic　condition.　The　results　showed　that　the　biomass　achieved　initial　granulation　successfully　(D　[4,　3]　=　280.44　and　346.28　mu　m)　in　both　CFR　and　SBR　on　day　70.　Compared　with　SBR,　a　better　performance　(0.33　kg　N　m(-3)　d(-1))　was　gotten　in　CFR　due　to　a　better　retention　capacity　of　biomass　(1397　mg　L-1),　when　seasonal　drop　of　water　temperature　occurred　(18-14　degrees　C).　Thus,　different　operations　led　to　different　granulation　styles　of　anammox.　Granules　in　CFR　had　better　rheological　properties　than　that　in　SBR.　Based　on　a　stable　and　suitable　environment　provided　by　CFR,　anaerobic　ammonium　oxidation　bacteria　(AnAOB)　are　able　to　self-aggregate　easily　and　secret　extracellular　polymeric　substances　(EPS),　which　can　capture　other　bacteria　as　home　guardians.　In　SBR,　AnAOB　live　inside　the　tan　granules　under　the　protection　of　other　bacteria　and　thick　EPS;　other　aggregations　stick　to　solid　carrier　surface　to　form　biofilm.　(C)　2021　Elsevier　B.V.　All　rights　reserved.