The　microstructure　and　micro-orientation　evolution　of　a　novel　high-temperature　titanium　alloy　were　characterized　during　a　two-step　deformation　process.　In　the　first　step,　the　specimens　were　deformed　at　temperatures　of　800,　850,　900　and　950　degrees　C,　and　subsequently　deformed　in　the　same　beta-phase　region　of　1050　degrees　C.　The　specimens　pre-strained　at　800　degrees　C　and　950　degrees　C　were　fully　recrystallized　during　the　beta-phase　deformation　process.　By　contrast,　the　specimens　pre-strained　at　850　and　900　degrees　C　were　not　fully　recrystallized　during　the　beta-phase　deformation　process.　This　indicates　that　the　extent　of　dynamic　recrystallization　did　not　increased　monotonically　with　the　pre-strain　temperature.　The　extent　of　dynamic　recrystallization　was　related　to　the　stored　energy　accumulated　in　the　alpha　and　beta-phase　during　the　pre-strain　deformation.　The　change　in　stored　energy　was　based　on　the　difference　in　microstructure　morphology　produced　by　different　pre　deformation　temperatures,and　stored　energy　was　quantitatively　estimated　based　on　the　local　misorientation　distribution.　Meanwhile,　the　generation　of　dynamically　recrystallized　grains　was　beneficial　to　decreasing　the　generation　possibility　of　the　macro-zone.　This　decrease　was　attributed　to　the　refinement　of　beta-grains　and　randomly　distributed　orientation,　which　was　also　related　to　the　decrease　in　the　typically　distributed　＂two　parallel　wings＂　orientation　between　the　alpha　and　beta　phase　induced　by　uncrystallized　deformation.　Additionally,　the　two-step　deformation　process　with　the　pre-strain　at　the　(alpha　+　beta)　middle　regime　was　an　effective　strategy　to　promote　the　dynamic　recrystallization　and　decrease　the　generation　probability　of　the　macro-zone.　(c)　2021　Elsevier　B.V.　All　rights　reserved.