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Author:

王聪 (王聪.) | 李秀 (李秀.) | 牛苗 (牛苗.) | 戴阳光 (戴阳光.) | 董哲岳 (董哲岳.) | 董小岩 (董小岩.) | 余双庆 (余双庆.) | 杨怡姝 (杨怡姝.)

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CSCD

Abstract:

目的:建立一种基于半数组织培养感染剂量(median tissue culture infective dose,TCID50)检测9型腺相关病毒(adeno-associated virus type 9,AAV9)载体制品感染性滴度的方法。方法:利用含AAV2 rep和cap基因的1型单纯疱疹病毒(herpes simplex virus type1,HSV1)做为辅助病毒与梯度稀释的AAV9载体制品共同感染HEK-293细胞,培养48 h后用实时荧光定量PCR(quantitative real-time PCR,q PCR)扩增AAV特异性反向末端重复序列(inverted termi...

Keyword:

感染性滴度 9型腺相关病毒载体 半数组织培养感染剂量

Author Community:

  • [ 1 ] 北京工业大学
  • [ 2 ] 北京五加和基因科技有限公司

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Source :

中国生物工程杂志

Year: 2021

Issue: 10

Volume: 41

Page: 28-32

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 9

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