Indexed by:
Abstract:
目的:建立一种基于半数组织培养感染剂量(median tissue culture infective dose,TCID50)检测9型腺相关病毒(adeno-associated virus type 9,AAV9)载体制品感染性滴度的方法。方法:利用含AAV2 rep和cap基因的1型单纯疱疹病毒(herpes simplex virus type1,HSV1)做为辅助病毒与梯度稀释的AAV9载体制品共同感染HEK-293细胞,培养48 h后用实时荧光定量PCR(quantitative real-time PCR,q PCR)扩增AAV特异性反向末端重复序列(inverted termi...
Keyword:
Reprint Author's Address:
Email:
Source :
中国生物工程杂志
Year: 2021
Issue: 10
Volume: 41
Page: 28-32
Cited Count:
WoS CC Cited Count: 0
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 9
Affiliated Colleges: