Partial-denitrification　coupled　with　ANAMMOX　is　a　novel　biological　nitrogen　removal　technology,　which　is　expected　to　significantly　reduce　the　external　carbon　source　dosage　for　advanced　nitrogen　removal　from　municipal　wastewater.　In　this　study,　ANAMMOX　sludge　was　inoculated　to　investigate　advanced　nitrogen　removal　performance　and　sludge　characteristics　in　a　partial-denitrification/ANAMMOX　reactor.　The　results　showed　that　inoculation　of　ANAMMOX　sludge　could　quickly　start　the　partial-denitrification/ANAMMOX　reactor.　The　effluent　total　nitrogen　concentrations　were　(4.82±1.84)　mg•L-1　with　a　chemical　oxygen　demand　of　2.19±0.08.　Sludge　particles　larger　than　0.20　mm　accounted　for　86.16%　in　the　reactor.　This　meant　that　granular　sludge　was　formed,　which　was　conducive　to　good　retention　of　ANAMMOX　bacteria　in　the　reactor.　The　external　carbon　source　dosage　and　the　oxygen　requirement　for　nitrification　can　be　reduced　by　applying　partial-denitrification　coupled　with　ANAMMOX　to　advanced　nitrogen　removal　from　the　effluent　of　secondary　clarifier　in　municipal　wastewater　treatment　plants.　©　2020,　Science　Press.　All　right　reserved.