With　combination　of　physical　experiment　and　numerical　simulation,　real-time　hybrid　simulation　(RTHS)　can　enlarge　the　dimensions　of　testing　specimens　and　improve　the　testing　accuracy.　However,　due　to　the　limitation　of　computing　capacity,　the　maximum　degrees　of　freedom　for　numerical　substructure　are　less　than　7000　from　the　reported　RTHS　testing.　It　cannot　meet　the　testing　requirements　for　evaluating　the　dynamic　performance　of　large　and　complex　engineering　structures.　Taking　advantages　of　parallel　computing　toolbox　(PCT)　in　Matlab　and　high-performance　computing　of　graphics　processing　unit　(GPU).　A　RTHS　framework　based　on　MATLAB　and　GPU　was　established　in　this　work.　Using　this　framework,　a　soil-structure　interaction　system　(SSI)　was　tested　by　a　shaking　table　based　RTHS.　Meanwhile,　the　dynamic　response　of　this　SSI　system　was　simulated　by　finite　element　analysis.　The　comparison　of　simulation　and　testing　results　demonstrated　that　the　proposed　testing　framework　can　implement　RTHS　testing　successfully.　Using　this　method,　the　maximum　degrees　of　freedom　for　numerical　substructure　can　reach　to　27,000,　which　significantly　enhance　the　testing　capacity　of　RTHS　testing　for　large　and　complex　engineering　structures.