The　intergeneric　conjugative　transfer　of　antibiotic　resistance　genes　(ARGs)　is　recognized　as　an　important　way　to　the　dissemination　of　antibiotic　resistance.　However,　it　is　unknown　whether　the　extensive　use　of　chloroxylenol　(para-chloro-meta-xylenol,　PCMX)　in　many　pharmaceutical　personal　care　products　will　lead　to　the　spread　of　ARGs.　In　this　study,　the　abilities　and　mechanisms　of　PCMX　to　accelerate　the　intergeneric　conjugative　transfer　were　investigated.　Results　showed　that　exposure　of　bacteria　to　environmental　concentrations　of　PCMX　(0.20-1.00　mg/L)　can　significantly　stimulate　the　increase　of　conjugative　transfer　by　8.45-9.51　fold.　The　phenotypic　experiments　and　genome-wide　RNA　sequencing　revealed　that　0.02-5.00　mg/L　PCMX　exposure　could　increase　the　content　of　alkaline　phosphatase　and　malondialdehyde,　which　are　characteristic　products　of　cell　wall　and　membrane　damage.　In　addition,　PCMX　could　lead　to　excessive　production　of　reactive　oxygen　species　(ROS)　by　1.26-2.00　times,　the　superoxide　dismutase　and　catalase　produced　by　bacteria　in　response　to　oxidative　stress　were　not　enough　to　neutralize　the　damage　of　ROS,　thus　promoting　the　conjugative　transfer.　Gene　Ontology　enrichment　analysis　indicated　that　cell　membrane　permeability,　pili,　some　chemical　compounds　transport　and　energy　metabolism-affected　conjugative　transfer.　This　study　deepened　the　understanding　of　PCMX　in　promoting　propagation　of　ARGs,　and　provided　new　perspectives　for　use　and　treatment　of　personal　care　products.　(c)　2021　Elsevier　B.V.　All　rights　reserved.