In　this　study,　intermittent　aeration　strategy　was　investigated　in　a　plug-flow　reactor　on　real　municipal　wastewater.　Over　200　days　of　operation,　the　total　inorganic　nitrogen　(TIN)　removal　efficiency　of　72.43　+/-　7.56%　was　achieved　with　a　total　aerobic　hydraulic　retention　time　in　the　range　2.4-3.0　h　under　a　low　C/N　ratio　of　3.19.　The　batch　tests　showed　that　the　activity　of　nitrite　oxidizing　bacteria　(NOB)　was　effectively　inhibited,　and　simultaneous　nitrifi-cation　and　denitrification　via　nitrite　were　observed　under　double　intermittent　aeration　mode.　The　Illumina　MiSep　sequencing　revealed　that　the　relative　abundance　of　the　Nitrospira　as　the　only　detected　NOB,　decreased　from　2.22%　(day　0)　to　0.91%　(day　207)　at　the　genus　level.　Overall,　this　study　provides　a　new　strategy　for　NOB　suppression　to　strengthen　nitrogen　removal　from　low　C/N　domestic　wastewater　through　the　continuous　process.