Part　causes　down　syndrome　or　all　the　third　copy　of　chromosome　21,　has　been　discovered　for　over　a　hundred　years.　Although　many　studies　have　been　carried　in　this　field　to　find　out　how　to　cure　the　patients　or　animals,　no　researchers　can　fully　treat　this　disease.　Thus,　this　research　manages　to　analyze　the　expression　level　of　the　proteins　encoded　by　the　genes　in　mice　with　down　syndrome　by　using　the　binary　logistic　regression　method.　The　consequence　shows　that　two　significant　proteins　are　affected　most　-　ITSN1_N　and　BRAF_N.　Behind　this,　the　predicted　data　are　carried　by　10-folds　cross-validation,　then　find　out　the　result　has　high　accuracy.　Moreover,　the　data　are　randomly　divided　into　20%,　40%,　60%,　and　80%　to　test　the　relationship　between　data　quantity　and　accuracy,　and　it　shows　that　the　more　data　amount,　the　more　precise　it　can　be.　©　2022　SPIE