Currently,　a　growing　number　of　biomaterials　have　been　evaluated　to　be　beneficial　to　the　application　of　neural　tissue　engineering.　However,　their　deficient　mechanical　and　electrical　properties　limit　their　further　application,　especially　for　nerve　regeneration.　Therefore,　the　combination　of　biological　matrix　and　conductive　materials　has　been　applied　to　meet　the　requirements　for　nerve　tissue　engineering.　In　this　work,　conductive　collagen　(COL)/multiwalled　carbon　nanotube　(MWNT)　composite　films　with　different　MWNT　concentrations　were　developed　by　the　solvent-evaporation　method.　The　effects　of　rigid　MWNT　on　the　structure,　mechanical,　thermal,　and　electrical　properties　of　the　flexible　COL-based　film　were　evaluated.　The　evaluation　of　mechanical　properties　revealed　that　the　tensile　strength　of　the　COL/MWNT　composite　films　was　almost　eight　times　as　high　as　that　of　the　pure　COL　film.　The　electrical　property　assessment　demonstrated　that　the　electrical　conductivity　of　COL/MWNT-0.25%　reached　0.45　S/cm,　meeting　the　electrical　stimulation　conditions　required　for　nerve　growth.　Furthermore,　the　cell　viability　assays　revealed　that　the　COL/MWNT　composite　films　were　non-cytotoxic　and　appropriate　for　cell　growth.　Our　work　proved　that　the　conductive　COL/MWNT　composite　films　exhibited　great　potential　for　nerve　tissue　engineering　application,　which　provided　a　novel　self-electrical　stimulated　platform　for　the　treatment　of　neural　injuries.　©　2022　Author(s).