Background:　Controlling　excess　biomass　accumulation　and　clogging　is　important　for　maintaining　the　performance　of　gas　biofilters　and　reducing　energy　consumption.　Interruption　of　bacterial　communication　(quorum　quenching)　can　modulate　gene　expression　and　alter　biofilm　properties.　However,　whether　the　problem　of　excess　biomass　accumulation　in　gas　biofilters　can　be　addressed　by　interrupting　bacterial　communication　remains　unknown.　Results:　In　this　study,　parallel　laboratory-scale　gas　biofilters　were　operated　with　Rhodococcus　sp.　BH4　(QQBF)　and　without　Rhodococcus　sp.　BH4　(BF)　to　explore　the　effects　of　quorum　quenching　(QQ)　bacteria　on　biomass　accumulation　and　clogging.　QQBF　showed　lower　biomass　accumulation　(109　kg/m(3))　and　superior　operational　stability　(85-96%)　than　BF　(170　kg/m(3);　63-92%)　at　the　end　of　the　operation.　Compared　to　BF,　the　QQBF　biofilm　had　lower　adhesion　strength　and　decreased　extracellular　polymeric　substance　production,　leading　to　easier　detachment　of　biomass　from　filler　surface　into　the　leachate.　Meanwhile,　the　relative　abundance　of　quorum　sensing　(QS)-related　species　was　found　to　decrease　from　67　(BF)　to　56%　(QQBF).　The　QS　function　genes　were　also　found　a　lower　relative　abundance　in　QQBF,　compared　with　BF.　Moreover,　although　both　biofilters　presented　aromatic　compounds　removal　performance,　the　keystone　species　in　QQBF　played　an　important　role　in　maintaining　biofilm　stability,　while　the　keystone　species　in　BF　exhibited　great　potential　for　biofilm　formation.　Finally,　the　possible　influencing　mechanism　of　Rhodococcus　sp.　BH4　on　biofilm　adhesion　was　demonstrated.　Overall,　the　results　of　this　study　achieved　excess　biomass　control　while　maintaining　stable　biofiltration　performance　(without　interrupting　operation)　and　greatly　promoted　the　use　of　QQ　technology　in　bioreactors.