Acesulfame　potassium　(ACE-K)　is　a　widely　utilized　sugar　substitute　with　increasing　demand,　which　is　frequently　de-tected　in　various　environmental　matrix　due　to　recalcitrance.　However,　a　general　consensus　on　the　contribution　of　ni-trifying　and　denitrifying　process　to　ACE-K　removal　is　lacking.　Therefore,　ACE-K　removal,　its　effects　on　antibiotic　resistant　genes　(ARGs)　propagation　and　microbial　community　in　nitrifying　sequencing　batch　reactor　(N-SBR)　and　denitrifying　sequencing　batch　reactor　(D-SBR)　inoculated　with　the　identical　activated　sludge　were　investigated.　In　this　study,　ACE-K　can　be　eliminated　in　N-SBR　with　satisfying　removal　efficiency　(96.76　+/-　8.33　%)　after　13　d　acclima-tion,　while　it　remained　persistent　(average　ACE-K　removal　efficiency　of　2.24　+/-　1.86　%)　in　D-SBR　during　84　d　expo-sure.　Moreover,　ACE-K　hardly　affected　the　performances　of　these　two　types　of　reactors　and　had　little　impact　on　nitrifying　and　denitrifying　functional　genes.　However,　initial　contact　with　ACE-K　would　increase　ARGs　abundance,　network　analysis　showed　functional　bacteria　in　each　reactor　were　possible　ARGs　hosts.　Potential　ACE-K　degrading　gen-era　Chelatococcus,　Bosea　and　Aquamicrobium　were　found　in　both　reactors.　LefSe　analysis　showed　that　Phyllobacteriaceae　containing　Aquamicrobium　genus　was　a　differentially　enriched　family　in　N-SBR.　This　research　might　provide　a　perspec-tive　for　better　understanding　factor　affecting　ACE-K　fate　in　wastewater　treatment　process　and　its　ecological　risks.