Functional　separators　have　played　important　roles　in　improving　the　electrochemical　performance　of　lithium-sulfur　(Li-S)　batteries　by　addressing　the　key　issues　of　both　the　sulfur　cathode　and　lithium　anode.　Compared　with　other　materials　that　are　used　for　separator　functionalization,　two-dimensional　(2D)　materials　with　atomic　layer　thickness　and　infinite　lateral　dimensions　feature　several　advantages　of　ultra-thin　laminate　structure,　remarkable　physical　properties　and　tunable　surface　chemistry,　which　show　potential　applications　in　separator　functionalization　towards　addressing　the　issues　of　both　the　shuttle　effect　and　formation　of　Li　dendrites　in　Li-S　batteries.　In　this　review,　the　unique　advantages　of　2D　materials　for　separator　functionalization　in　Li-S　batteries　and　their　common　construction　methods　are　introduced.　Then,　recent　progress　and　advances　in　the　construction　of　2D　materials　functional　separators　are　summarized　in　detail　towards　inhibiting　the　shuttle　effect　of　polysulfides　and　suppressing　Li　dendrite　growth　in　Li-S　batteries.　Finally,　some　opportunities　and　challenges　of　2D　materials　for　constructing　high-performance　functional　separators　are　proposed.　We　anticipate　that　this　review　will　provide　new　insights　into　separator　functionalization　for　developing　advanced　Li-S　batteries.