Different　flow　channel　structures　of　unitized　regenerative　fuel　cells　have　great　effects　on　the　flow　process　of　reactive　gas,　liquid　product　and　flowing　purge　gas　inside　channels　and　the　transport　process　of　them　in　the　porous　media　layer.　In　this　study,　the　unitized　regenerative　fuel　cell　with　three　different　kinds　of　channel　structures　are　designed　and　manufactured　based　on　the　numerical　simulation.　By　utilizing　the　unitized　regenerative　fuel　cell　testing　set-up,　the　output　electricity　performance　test　of　the　single　cell　in　the　fuel　cell　mode,　electrolytic　cell　mode　and　mode　switching　process　are　realized,　and　effective　verification　data　are　provided　for　the　two-phase　model　verification　of　unitized　regenerative　fuel　cells.　The　results　show　that　different　channel　configurations　result　in　various　performance　behaviors　during　different　procedures　of　the　cell.　Using　the　channel　having　1　mm　square　cross-section　structures　can　perform　better　start-up　performance,　compared　to　the　T-wide　and　T-narrow　section　channels.　When　entering　into　electrolysis　cell　mode,　compared　to　the　cell　with　T-section　channels,　the　output　performance　of　the　cell　constructed　with　1　mm　square　section　channel　is　decreased　obviously　at　the　initial　stage　of　start-up　because　of　the　residual　water　affecting.　The　present　experiments　combined　with　simulation　works　facilitate　better　understanding　how　the　performance　and　two-phase　species　changing　at　the　operating　procedures　of　unitized　regenerative　fuel　cells　with　different　flow　channels.　©　2023　Elsevier　Ltd