Mature　aerobic　granular　sludge　was　inoculated　into　the　SBR　reactor　at　room　temperature,　and　the　actual　domestic　sewage　was　used　as　the　influent　matrix,　and　the　A/O/A　time　ratio　strategy　was　combined　with　gradient　feeding　to　improve　the　denitrification　and　phosphorus　removal　effect　of　the　sludge.　When　the　anoxic　time　was　extended　from　0.5h　to　1.5h,　the　efficient　denitrification　and　phosphorus　removal　can　be　ensured　at　the　same　time.　At　this　time,　the　A/O/A　time　ratio　was　1:1.6:1　which　made　the　average　TN　increase　to　81.27%,　the　average　TP　removal　rate　to　above　90%,　and　the　average　COD　removal　rate　to　above　85%.　Meanwhile,　the　simultaneous　nitrification　and　denitrification　(SND)　rate　decreased　to　25.24%,　and　the　ratio　of　anoxic　and　phosphorus　uptake　increased　from　0.43%　to　2.81%,　which　further　indicated　that　the　denitrification　contribution　of　denitrifying　sugar　bacteria　(DGAOs)　to　denitrification　increased　at　this　A/O/A　time　ratio.　When　the　anoxic　time　was　extended　to　2h　and　the　A/O/A　time　ratio　was　3:5:4,　excessive　hypoxia　led　to　the　endogenous　respiration　of　microorganisms　and　the　reproduction　of　filamentous　bacteria.　The　average　removal　rates　of　TN　and　TP　decreased　to　51.48%　and　72.46%,　respectively.　When　the　anoxic　time　was　1.5h,　the　MLSS　was　3501mg/L　which　was　lower　but　the　MLVSS/MLSS(f)　increased　to　0.95,　indicating　that　the　non-functional　bacteria　were　washed　away.　The　bacteria　with　nitrogen　and　phosphorus　removal　functions　can　grow　in　large　quantities.　With　an　extension　of　the　anoxic　time　to　1.5h　(or　the　A/O/A　time　ratio　is　1:1.6:1),　the　PN/PS　increases　to　6.89,　so　the　stability　of　the　particles　was　continuously　enhanced.　The　extension　of　the　hypoxia　time　to　within　1h　had　a　greater　impact　on　LB-EPS　and　EPS　but　little　effect　on　TB-EPS.　The　hypoxia　time　more　than　1h　had　a　greater　effect　on　TB-EPS　and　can　make　EPS　increase　to　126.16mg/g.　When　the　hypoxia　time　was　extended　to　2h,　the　EPS　content　increased　to　479.92mg/g　due　to　the　massive　death　of　microbes　that　intake　PN　and　PS　released　from　endogenous　respiration　of　microorganisms.　©　2022　Chinese　Society　for　Environmental　Sciences.　All　rights　reserved.