Adding　rejuvenator　is　a　feasible　method　to　restore　the　properties　of　aged　asphalt.　Due　to　the　complex　rejuve-nation　mechanism　between　different　rejuvenators　and　aged　asphalt,　there　is　no　unified　standard　to　evaluate　the　rejuvenation　effect　of　rejuvenator.　Most　of　the　current　research　on　rejuvenator　focus　on　the　characterization　of　macro　mechanical　properties　of　rejuvenated　asphalt　binder.　In　order　to　explore　the　changes　of　chemical　composition　and　micromechanical　properties　of　asphalt　during　aging　and　rejuvenation,　fifteen　kinds　of　virgin　asphalt　samples　at　different　aging　and　rejuvenation　stages　were　prepared.　A　commercial　rejuvenator,　aromatic　oil　and　warm　mix　agent　were　selected　as　rejuvenators.　The　fifteen　asphalt　samples　involved　various　aging　methods,　including　water-ultraviolet　aging,　water　free　ultraviolet　aging,　RTFOT　and　PAV.　Through　gel　perme-ation　chromatography　(GPC),　Fourier　transform　infrared　spectrometer　(FTIR)　and　atomic　force　microscope　(AFM)　test,　the　reversibility　evaluation　index　Rw,　RC=O,　RS=O　and　RDMT　were　proposed.　These　indexes　could　be　used　to　quantitatively　evaluate　the　rejuvenation　effects　of　rejuvenator　on　the　specific　properties　of　aged　asphalt.　Based　on　the　linear　fitting　results　among　various　indexes,　micro　reversibility　index　of　virgin　asphalt　(IRIV)　was　proposed.　The　results　showed　that　the　commercial　rejuvenator　and　warm　mix　agent　had　a　limited　effect　on　the　recovery　of　chemical　composition　and　molecular　weight　of　aged　asphalt.　The　commercial　rejuvenator　over　restored　the　micro　mechanical　properties　of　aged　asphalt.　The　effect　of　aromatic　oil　on　micro　mechanical　properties　of　aged　asphalt　was　between　commercial　rejuvenator　and　warm　mix　agent.　This　study　provides　a　way　to　quantitatively　evaluate　the　rejuvenation　effect　of　rejuvenator　on　virgin　asphalt　binder.　It　is　helpful　to　better　understand　the　aging-rejuvenation　process　of　virgin　asphalt　binder　more　comprehensively　from　chemical　and　micromechanical　properties.