Peroxynitrite　(ONOO-)　is　a　crucial　reactive　oxygen　species　that　plays　a　vital　role　in　cellular　signal　transduction　and　homeostatic　regulation.　Determining　and　visualizing　peroxynitrite　accurately　in　biological　systems　is　important　for　understanding　its　roles　in　physiological　and　pathological　activity.　Among　the　various　detection　methods,　fluorescent　probe-based　spectroscopic　detection　offers　real-time　and　minimally　invasive　detection,　high　sensitivity　and　selectivity,　and　easy　structural　and　property　modification.　This　review　categorizes　fluorescent　probes　by　their　fluorophore　structures,　highlighting　their　chemical　structures,　recognition　mechanisms,　and　response　behaviors　in　detail.　We　hope　that　this　review　could　help　trigger　novel　ideas　for　potential　medical　diagnostic　applications　of　peroxynitrite-related　molecular　diseases.