• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
搜索

Author:

孙永军 (孙永军.) | 王湛 (王湛.) (Scholars:王湛) | 侯磊 (侯磊.) | 朱可欣 (朱可欣.) | 杜心培 (杜心培.)

Indexed by:

incoPat zhihuiya

Abstract:

一种快速检测食品中沙门氏菌的方法,涉及食品检测技术领域。用PCR方法对待测食品样品中沙门氏菌的inVA基因进行检测,包括以下步骤:将待测食品样品加入缓冲蛋白胨水中增菌,然后用生理盐水洗菌后煮沸裂解细胞,离心取上清液;以获得的上清液为PCR反应模板,并利用沙门氏菌inVA基因的检测引物进行PCR检测;其中,沙门氏菌inVA基因的检测引物为:上游引物inVAF:5’‑tcctccgctctgtctactta‑3’;下游引物inVAR:5’‑accgaaatattcattgacgtt‑3’。本发明方法的检测结果与传统培养方法和系统检测结果一致,检出限为100cfu/25g,准确性达100%。

Keyword:

Reprint Author's Address:

Email:

Show more details

Related Keywords:

Related Article:

Patent Info :

Type: 发明申请

Patent No.: CN201711478860.6

Filing Date: 2017-12-29

Publication Date: 2018-06-26

Pub. No.: CN108203736A

Applicants: 北京工业大学

Legal Status: 驳回

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 9

Online/Total:242/10523782
Address:BJUT Library(100 Pingleyuan,Chaoyang District,Beijing 100124, China Post Code:100124) Contact Us:010-67392185
Copyright:BJUT Library Technical Support:Beijing Aegean Software Co., Ltd.