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Author:

冯继宏 (冯继宏.) | 童石渊 (童石渊.) | 於然 (於然.) | 白桦娟 (白桦娟.)

Indexed by:

incoPat zhihuiya

Abstract:

一种可用于微滴数字PCR的微滴制备方法,配制PCR水相,PCR水相包括上游引物、下游引物、PCR模板、PCRmix、以及双蒸水;将上述药品以此均匀混合,作为反应水相备用;将上游引物、下游引物、PCR模板、PCRmix依次均匀混合,滴加双蒸水后将溶液体系作为反应水相备用;配制PCR油相,将丙三醇、Trition X‑100、Tween‑80、壬基苯基醚IgelCO520依次均匀混合;取1体积水相PCR加到2体积的PCR油相中,涡旋搅拌并反应,得到油包水微滴;本发明提供的制备方法,可便捷地配制具有颗粒均匀、热力学性能稳定的油包水反应微滴,简便了微滴数字PCR实验的操作。

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Patent Info :

Type: 发明授权

Patent No.: CN201510267209.9

Filing Date: 2015-05-24

Publication Date: 2017-10-10

Pub. No.: CN104846100B

Applicants: 北京工业大学

Legal Status: 未缴年费

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 4

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