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Author:

冯继宏 (冯继宏.) | 童石渊 (童石渊.) | 於然 (於然.) | 白桦娟 (白桦娟.)

Indexed by:

incoPat zhihuiya

Abstract:

一种利用硅油的适用于微滴数字PCR的微滴制备方法,配制PCR水相,将上游引物、下游引物、PCR模板、PCRmix依次均匀混合,滴加双蒸水使溶液体系并分装3个管,各管体积相同并作为反应水相备用;配制PCR油相,取二甲基硅氧烷作为反应油相备用;取水相PCR加到油相中,涡旋震荡使油水两相充分乳化,得到油包水微滴;本发明提供的制备方法,可便捷地配制一种具有颗粒均匀、热力学性能稳定的油包水反应微滴,简便了微滴数字PCR实验的操作。

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Patent Info :

Type: 发明申请

Patent No.: CN201510267908.3

Filing Date: 2015-05-24

Publication Date: 2015-09-23

Pub. No.: CN104928368A

Applicants: 北京工业大学

Legal Status: 驳回

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 3

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