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Abstract:
一种利用硅油的适用于微滴数字PCR的微滴制备方法,配制PCR水相,将上游引物、下游引物、PCR模板、PCRmix依次均匀混合,滴加双蒸水使溶液体系并分装3个管,各管体积相同并作为反应水相备用;配制PCR油相,取二甲基硅氧烷作为反应油相备用;取水相PCR加到油相中,涡旋震荡使油水两相充分乳化,得到油包水微滴;本发明提供的制备方法,可便捷地配制一种具有颗粒均匀、热力学性能稳定的油包水反应微滴,简便了微滴数字PCR实验的操作。
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Patent Info :
Type: 发明申请
Patent No.: CN201510267908.3
Filing Date: 2015-05-24
Publication Date: 2015-09-23
Pub. No.: CN104928368A
Applicants: 北京工业大学
Legal Status: 驳回
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ESI Highly Cited Papers on the List: 0 Unfold All
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30 Days PV: 3
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