Terahertz　metamaterial　technology,　as　an　efficient　nondestructive　testing　method,　has　shown　great　development　potential　in　the　detection　of　biological　samples.　In　this　study,　we　have　proposed　a　graphene-integrated　gold　nanoparticles　(AuNPs)　terahertz　(THz)　biosensor　and　have　analyzed　the　sensing　performance　of　four　IC-type　resonators　(ICSRs)　for　biosensors:　bare　ICSRs,　ICSRs-integrated　AuNPs,　ICSRs-integrated　graphene,　and　ICSRs-integrated　graphene　and　AuNPs.　The　AuNPs　generate　charge　accumulation　and　induce　local　electric　field　enhancement　due　to　the　tip　effect,　and　enhance　the　interaction　between　the　THz　wave　and　the　analyte,　thus　improving　the　sensitivity　of　the　sensor.　The　combination　of　AuNPs　and　ICSRs-integrated　graphene　results　in　sensors　with　more　pronounced　amplitude　changes　and　higher　sensitivity.　Due　to　the　Fermi　energy　level　of　graphene　being　close　to　the　Dirac　point,　the　sensor　is　ultra-sensitive　to　external　stimuli　with　a　limit　of　detection　of　10.48　fg/ml　for　aspartic　acid　solutions.　This　biosensor　has　the　potential　to　detect　trace　amino　acids　with　high　sensitivity,　contributing　to　early　disease　detection　and　environmental　detection　for　early　diagnosis　and　ecological　balance.　©　2023　Elsevier　B.V.