In　this　work,lithium　(Li)-aluminum　(Al)　layered　double　hydroxide　(LDH)　films　modified　by　4-amino-2-((hydrazine　methylene)　amino)-4-oxobutanoic　acid　(denoted　as　AOA　acid)　and/or　1H,1H,2H,2H-perfluorooctyltriethoxysilane　were　prepared　on　6N01　Al　alloy　by　a　facile,in-situ　growth　method　with　enhanced　hydrophobicity,anti-biofouling　and　anti-corrosion　performance.The　preparation　is　low　energy　consumptive　and　environment　friendly,relying　on　self-assembly　at　ambient　temperature.The　structure,molecular　weight　and　functional　groups　of　the　synthesized　AOA　acid　were　characterized　by　NMR　spectrometer,ESI-MS　spectrometer　and　Fourier　transform　infrared　(FT-IR)　spectroscopy.And　the　compositions,structure　and　morphology　of　the　films　were　characterized　by　Fourier　transform　infrared(FT-IR)　spectroscopy,glancing-angle　X-ray　diffraction　(GA-XRD),X-ray　photoelectron　spectroscopy　(XPS),field　emission　scanning　electron　microscopy　(FE-SEM)　and　energy-dispersive　x-ray　spectrum　(EDS).Water　contact　angle　measurements　(CA)　and　atomic　force　microscopy　(AFM)　characterization　show　that　the　films　possess　a　micro/nanostructure　with　an　improved　hydrophobicity.Immersion　test,neutral　salt　tests　(NSS)　and　electrochemical　impedance　spectroscopy　(EIS)　conducted　in　3.5　wt.％　NaCl　solutions　demonstrate　the　improved　corrosion　resistance　of　the　films　over　bare　Al　alloy.Meanwhile,the　films　also　possess　an　excellent　anti-bacterial　property　to　Escherichia　coli,Bacillus　subtilis　and　sulfate-reducing　bacteria.