Outdoors　the　living　waste　water　after　using　A/O　removes　phosphorus　was　taken　as　matrix　to　start　the　biofilter　of　CANON　process,　aimed　at　the　problem　that　nitrite　oxidizing　bacteria　(NOB)　excesses　proliferation　in　CANON　process　and　causes　the　effluent　nitrogen　concentration　to　exceed　the　first　level　A　discharge　standard　of　pollutants　for　municipal　wastewater　treatment　plant,　to　experiment　and　investigate　the　effect　of　high　ammonia　loading　rate,　back　washing　and　anaerobic　operation　on　the　NOB　in　the　filter　column.　The　results　show　that　the　NOB　had　adaptability　to　the　high　ammonia　loading　rate,　when　the　influent　ammonia　nitrogen　loading　rate　was　0.60kg/(m3·d),　after　the　continuous　operation　of　80d,　the　total　nitrogen　removal　rate　was　stable　at　65%　or　so,　the　maximum　effluent　total　nitrogen　concentration　was　15.8mg/L,　exceeding　the　first　level　A　emission　standards;　NOB　was　eluted　in　CANON　process　by　the　means　of　reverse　washing,　after　the　back　washing　the　total　nitrogen　concentration　of　the　continuous　40d　effluent　was　less　than　10.5mg/L;　the　inhibitory　effect　of　anaerobic　operation　on　NOB　was　larger　but　its　inhibitory　effect　on　AOB　was　smaller,　from　after　anaerobic　operation　recovers　low　DO　stable　operation,　the　total　nitrogen　removal　rate　above　continuous　40d　was　greater　than　80%,　and　the　maximum　effluent　total　nitrogen　concentration　was　9.5mg/L.　©　2017,　Editorial　Board　of　China　Environmental　Science.　All　right　reserved.