Eco-lanes　are　an　important　means　of　implementing　eco-driving　behavior　and　reducing　vehicle　emissions.　Cooperative　vehicle　infrastructure　connected　technology　offers　further　energy　savings　and　emission　reduction　benefits　through　eco-lanes.　Based　on　the　cooperative　vehicle　infrastructure　driving　simulator　experiment　platform,　40　subjects　were　selected　to　carry　out　the　comparative　test　of　vehicle　energy　consumption　emissions　under　three　conditions　of　expressways:　ordinary　road,　eco-lane,　and　vehicle-mounted　human-machine　interaction　system　(HMI)　based　eco-lane.　Results　showed　that　compared　with　ordinary　road　sections,　the　eco-lane　offers　significant　energy　savings　and　emission　reduction　potential,　which　can　reach　24.02%-59.04%　and　24.15%,　respectively.　Under　the　condition　of　cooperative　vehicle　infrastructure,　eco-lanes　have　the　highest　energy　saving　and　emission　reduction　potential,　with　the　emission　reduction　potential　of　24.46%-59.72%　and　energy　saving　potential　of　24.61%.　Vehicle　energy　consumption　and　emissions　can　be　significantly　reduced　by　setting　eco-lanes　in　main　expressway　sections　under　the　condition　of　cooperative　vehicle　infrastructure.　©　ASCE.