To　shorten　the　start-up　time　of　CANON　process　and　guide　the　sludge　dischargement　in　the　practical　project,　the　start-up　of　CANNON　process　and　influence　of　different　flocs　SRT　were　studied　by　inoculation　ANAMMOX　granules　of　different　proportions　in　two　same　specifications　of　SBR　reactor.　During　the　experiment,　the　temperature　was　controlled　at　30±1　and　pH　was　7-8.　The　results　showed　that　it　was　not　good　for　CANON　process　rapid　start-up　when　the　two　reactors　were　inoculated　with　5%　and　10%　ANAMMOX　granules　respectively　and　the　initial　FA　concentration　exceeded　44　mg·L-1.　When　the　two　reactors　were　inoculated　with　5%　and　10%　ANAMMOX　granular　sludge,　the　reactors　were　started-up　successfully　on　Day　46　and　Day　35,　respectively.　When　the　flocs　SRT　was　maintained　at　30　d,　90　d　and　un-initiative　discharge　respectively,　the　average　total　nitrogen　removal　rate　could　reach　0.35　kg·(m3·d)-1　stably.　The　quantitative　PCR　results　of　the　three　different　flocs　SRT　showed　that　with　increasing　flocs　SRT,　the　abundance　of　AOB　was　relatively　stable,　the　abundance　of　ANAMMOX　increased　slightly,　while　the　abundance　of　NOB　significant　increased.　Therefore,　it　is　necessary　to　elutriate　flocs　from　reactor　for　decreasing　the　quantity　of　NOB　and　long-term　stable　operation　of　the　CANON　process.　©　2017,　Science　Press.　All　right　reserved.