Hrr25　is　a　member　of　the　casein　kinase　1　family　in　Saccharomyces　cerevisiae　and　has　serine　/　threonine　protein　kinase　activity.　It　can　function　by　phosphorylating　a　variety　of　proteins.　The　substrate　proteins　of　Hrr25　include　autophagy　related　proteins,　COPII　(coat　protein　complexes　II)　vesicle　coat　proteins　Sec24　and　Sec23,　eukaryotic　translation　initiation　factor　6,　γ-　Tubulin　tub4,　and　extender　complex　protein　1,　etc.　In　addition,　Hrr25　can　also　interact　with　meiotic　recombinant　protein　Rec8,　Nucleoporin　Nup53,　transcription　regulator　Crz1,　and　transcription　activator　Haa1,　etc.　A　variety　of　interacting　proteins　of　Hrr25　enable　it　to　play　a　role　in　autophagy,　vesicular　transport,　microtubule　assembly,　meiosis,　mitosis,　DNA　repair,　ribosomal　biogenesis,　weak　organic　acid　stress　and　other　biological　processes.　In　order　to　better　understand　the　action　mechanism　of　Hrr25　in　various　biological　processes　and　the　relationship　between　various　biological　processes,　this　paper　summarizes　the　biological　functions　and　action　mechanism　of　Hrr25,　and　the　potential　significance　of　its　research,　so　as　to　provide　a　theoretical　basis　for　the　further　research　of　Hrr25.　©　2023　Chinese　Society　of　Biochemistry　and　Molecular　Biology,　Peking　University.　All　rights　reserved