Incorporating　zwitterionic　materials　into　polymer　matrix　is　an　effective　method　to　fabricate　high-performance　ultrafiltration　membranes,　which　can　improve　the　surface　hydrophilicity　while　optimizing　the　internal　structure　of　the　membrane.　In　this　study,　a　series　of　zwitterionic　copolymers,　4VP-MPC,　were　synthesized　using　4-vinylpyridine　(4VP)　and　2-methylacryloxyethyl　phosphocholine　(MPC),　and　their　impact　on　the　phase　inversion　process　of　polysulfate　(PSE)　was　investigated　thoroughly　by　changing　the　addition　amount　and　altering　the　copolymer　composition.　The　addition　of　4VP-MPC　accelerated　the　phase　inversion　rate,　reduced　the　skin　layer　thickness　and　improved　the　membrane　porosity.　Moreover,　the　hydrophilicity　of　the　membrane　surface　is　enhanced　due　to　the　segregation　of　the　zwitterionic　copolymer　during　the　membrane　formation　process.　A　novel　PSE/4VP-MPC　membrane　with　good　separation　performance　and　antifouling　property　was　fabricated,　with　a　pure　water　flux　of　221　L/(m2　h　bar),　a　BSA　rejection　rate　of　93.3　%,　and　a　flux　recovery　rate　of　94.6　%.　Additionally,　when　filtering　the　mixed　simulated　dialysate,　the　optimized　membrane　showed　clearance　rates　of　71.3　%　and　97.0　%　for　lysozyme　and　urea　respectively,　while　maintaining　a　high　BSA　rejection.　This　PSE/4VP-MPC　blend　membrane　demonstrated　potential　applications　in　the　field　of　ultrafiltration,　including　hemodialysis.　©　2024　Elsevier　B.V.