We　demonstrate　light-triggered　dynamic　covalent　assembly　of　a　linear　short　tetrapeptide　containing　two　terminal　cysteine　residues　in　an　AND　logic　manner.　A　photobase　generator　is　introduced　to　accomplish　light-mediated　pH　regulation　to　increase　the　reduction　potential　of　thiols　in　the　tetrapeptide,　which　activates　its　oxidative　polymerization　through　disulfide　bonds.　Interestingly,　it　is　elucidated　that　under　light　irradiation,　mere　co-existence　of　photobase　generator　and　the　oxidizing　agent　permits　the　polymerization　performance　of　this　tetrapeptide.　Hence,　a　light-triggered　AND　logic　dynamic　covalent　assembly　of　a　tetrapeptide　is　achieved.　Further,　upon　redox　response,　the　reversible　aggregation　and　disaggregation　can　be　transformed　for　numerous　times　due　to　the　dynamic　covalent　feature　of　disulfide　bond.　As　a　comparison,　no　assembly　occurs　for　a　short　peptide　containing　one　terminal　cysteine　residue　under　the　same　stimuli　condition.　This　work　offers　a　new　approach　to　remotely　control　programmable　molecular　assembly　of　short　linear　peptides　based　on　dynamic　covalent　bond,　holding　great　potential　in　wide　bioapplications.　©　2024　Elsevier　B.V.