Background:　Post-traumatic　stress　disorder　(PTSD)　refers　to　a　chronic　impairing　psychiatric　disorder　occurring　after　exposure　to　the　severe　traumatic　event.　Studies　have　demonstrated　that　medicinal　cannabis　oil　plays　an　important　role　in　neuroprotection,　but　the　mechanism　by　which　it　exerts　anti-PTSD　effects　remains　unclear.　Methods:　The　chronic　complex　stress　(CCS)　simulating　the　conditions　of　long　voyage　stress　for　4　weeks　was　used　to　establish　the　PTSD　mice　model.　After　that,　behavioral　tests　were　used　to　evaluate　PTSD-like　behaviors　in　mice.　Mouse　brain　tissue　index　was　detected　and　hematoxylin-eosin　staining　was　used　to　assess　pathological　changes　in　the　hippocampus.　The　indicators　of　cell　apoptosis　and　the　BDNF/TRPC6　signaling　activation　in　the　mice　hippocampus　were　detected　by　western　blotting　or　real-time　quantitative　reverse　transcription　PCR　experiments.　Results:　We　established　the　PTSD　mice　model　induced　by　CCS,　which　exhibited　significant　PTSD-like　phenotypes,　including　increased　anxiety-like　and　depression-like　behaviors.　Medicinal　cannabis　oil　treatment　significantly　ameliorated　PTSD-like　behaviors　and　improved　brain　histomorphological　abnormalities　in　CCS　mice.　Mechanistically,　medicinal　cannabis　oil　reduced　CCS-induced　cell　apoptosis　and　enhanced　the　activation　of　BDNF/　TRPC6　signaling　pathway.　Conclusions:　We　constructed　a　PTSD　model　with　CCS　and　medicinal　cannabis　oil　that　significantly　improved　anxiety-like　and　depressive-like　behaviors　in　CCS　mice,　which　may　play　an　anti-PTSD　role　by　stimulating　the　BDNF/TRPC6　signaling　pathway.