The　heterotrophic　nitrification-aerobic　denitrification　(HNAD)　strain　Exiguobacterium　H1　(H1)　was　isolated　in　this　study.　The　changes　in　nitrogen　metabolism　functions　of　H1　strain　were　discussed　in　presence　of　disinfectants　chloroxylenol　(PCMX)　and　benzethonium　chloride　(BEC)　alone　and　combined　pollution　(PCMX+BEC).　The　H1　strain　could　use　NH4+-N,　NO2--N　and　NO3--N　as　nitrogen　sources　and　had　good　nitrogen　removal　performance　under　conditions　of　C/N　ratio　25,　pH　5–8,　25–35　oC　and　sodium　acetate　as　carbon.　PCMX　and　BEC　alone　exhibited　hormesis　effects　on　H1　strain　which　promoted　the　growth　of　H1　strain　at　low　concentrations　but　inhibited　it　at　high　concentrations,　and　combined　pollution　showed　synergistic　inhibitory　on　H1　strain.　H1　strain　owned　a　full　nitrogen　metabolic　pathway　according　to　functional　genes　quantification.　PCMX　encouraged　nitrification　process　of　H1,　while　BEC　and　combined　pollution　mostly　blocked　nitrogen　removal.　PCMX,　but　not　BEC,　mainly　led　to　the　enrichment　of　resistance　genes.　These　findings　will　aid　in　systematic　assessment　of　contaminant　tolerance　characteristics　of　HNAD　strain　and　its　application　prospects.　©　2024　Elsevier　B.V.