In　this　paper,　a　seismic　and　vibration　reduction　measure　of　subway　station　is　developed　by　setting　a　segmented　isolation　layer　between　the　sidewall　of　structure　and　the　diaphragm　wall.　The　segmented　isolation　layer　consists　of　a　rigid　layer　and　a　flexible　layer.　The　rigid　layer　is　installed　at　the　joint　section　between　the　structural　sidewall　and　slab,　and　the　flexible　layer　is　installed　at　the　remaining　sections.　A　diaphragm　wall-segmented　isolation　layer-subway　station　structure　system　is　constructed.　Seismic　and　vibration　control　performance　of　the　diaphragm　wall-segmented　isolation　layer-subway　station　structure　system　is　evaluated　by　the　detailed　numerical　analysis.　Firstly,　a　three-dimensional　nonlinear　time-history　analysis　is　carried　out　to　study　the　seismic　response　of　the　station　structure　by　considering　the　effect　of　different　earthquake　motions　and　stiffness　of　segmented　isolation　layer.　Subsequently,　the　vibration　response　of　site　under　training　loading　is　also　studied　by　considering　the　influence　of　different　train　velocities　and　stiffness　of　the　segmented　isolation　layer.　Numerical　results　demonstrate　that　the　diaphragm　wall-segmented　isolation　layer-subway　station　structure　system　can　not　only　effectively　reduce　the　lateral　deformation　of　station　structure,　but　also　reduce　the　tensile　damage　of　the　roof　slab.　On　the　other　hand,　the　developed　reduction　measure　can　also　significantly　reduce　the　vertical　peak　displacements　of　site　under　training　loading.