Achieving　an　efficient　method　to　separate　cis–trans　isomers　of　trans-1,4-cyclohexanedicarboxylic　acid　remains　a　formidable　challenge　within　the　chemical　industry.　Here,　we　have　synthesized　a　Co(II)-based　dynamic　metal–organic　framework　material　involving　the　coordination　of　1,4-cyclohexanedicarboxylic　acid.　Single-crystal　X-ray　diffraction　and　high-performance　liquid　chromatography　(HPLC)　analysis　confirmed　that　only　pure　trans-1,4-cyclohexanedicarboxylic　acid　was　present　in　all　synthesized　crystals,　a　characteristic　that　can　be　utilized　for　the　separation　of　the　cis-　and　trans-isomers　of　1,4-cyclohexanedicarboxylic　acid.　Moreover,　the　MOF　crystals　displayed　reversible　structural　changes　and　were　capable　of　releasing　the　pure　trans-isomer　through　ion　exchange　with　a　salt　solution,　underscoring　their　potential　for　applications　in　molecular　separation.　©　2024　Elsevier　B.V.