Non-invasive　medical　nanofiber　technology,　characterized　by　its　high　specific　surface　area,　biocompatibility,　and　porosity,　holds　significant　potential　in　various　medical　domains,　including　tissue　repair　and　biosensing.　It　is　increasingly　becoming　central　to　healthcare　by　offering　safer　and　more　efficient　treatment　options　for　contemporary　medicine.　Numerous　studies　have　explored　non-invasive　medical　nanofibers　in　recent　years,　yet　a　comprehensive　overview　of　the　field　remains　lacking.　In　this　paper,　we　provide　a　comprehensive　summary　of　the　applications　of　electrospun　nanofibers　in　non-invasive　medical　fields,　considering　multiple　aspects　and　perspectives.　Initially,　we　introduce　electrospinning　nanofibers.　Subsequently,　we　detail　their　applications　in　non-invasive　health,　including　health　monitoring,　personal　protection,　thermal　regulation,　and　wound　care,　highlighting　their　critical　role　in　improving　human　health.　Lastly,　this　paper　discusses　the　current　challenges　associated　with　electrospun　nanofibers　and　offers　insights　into　potential　future　development　trajectories.