Visualizing　RNA　is　critical　for　understanding　RNA　expression　patterns　and　spatial　organization　within　cells,　offering　valuable　insights　into　gene　regulation　and　cellular　functions.　High-resolution　RNA　imaging　techniques　are　therefore　indispensable　for　revealing　the　complexities　of　cellular　pathways　and　physiological　processes.　Traditional　RNA　imaging　methods,　however,　face　significant　limitations,　such　as　high　background　noise　resulting　from　labeling　or　cell　fixation,　which　can　impede　the　accurate　tracking　of　RNA　dynamics　in　live　cells.　Fluorescent　light-up　RNA　aptamers　(FLAPs)　have　emerged　as　a　powerful　alternative,　distinguished　by　their　capacity　for　enhanced　fluorescence　activation,　reduced　background　interference,　and　advantages　such　as　label-free　imaging,　small　molecular　size,　and　customizable　structures.　In　this　review,　we　provide　an　overview　of　the　development　of　FLAPs,　explore　recent　advancements　in　FLAP-based　RNA　imaging　strategies,　and　discuss　both　the　challenges　and　future　directions　in　the　field.　Through　this　analysis,　we　aim　to　facilitate　the　further　development　and　application　of　FLAPs　in　RNA　research,　fostering　innovation　and　offering　new　perspectives　in　the　study　of　RNA　biology.