In　recent　years,　piezoelectric　functional　nanofibers　have　been　widely　used　in　vascular　tissue　engineering.　The　preparation　of　nanofibers　with　good　piezoelectric　properties　is　beneficial　for　exploring　the　effects　of　mechanical　signals　induced　electrical　signals　on　cell　behavior.　The　polylactic　acid　@　polyethylene　oxide　@　barium　titanate　(PLA　@　PEO　@BT)　piezoelectric　functional　nanofiber　successfully　was　prepared　by　electrospinning　blending　method.　The　nanofibers　were　characterized　for　their　surface　morphology,　hydrophilicity,　composition,　mechanical　properties,　and　piezoelectric　properties　using　scanning　electron　microscopy,　contact　angle　analyzer,　X-ray　diffractometer,　ultra-high　precision　static　micro　force　testing　machine,　and　oscilloscope.　PLA　and　PEO　with　different　ratios　in　PLA　@　PEO　@　BT　fibers　formed　a　staggered　porous　network　fiber　structure　and　possessed　different　piezoelectric　properties.　When　PLA:　PEO=1:0,　the　output　voltage　was　the　highest,　at　0.630V.　As　the　proportion　of　PLA　decreased,　the　output　voltage　was　the　lowest　at　0.014V　when　PLA:　PEO=1:1.　Subsequently,　due　to　the　formation　of　numerous　hydrogen　bonds　between　the　hydroxyl　groups　of　PEO　and　BT,　the　output　voltage　gradually　increased　as　the　proportion　of　PEO　increased.　When　PLA:　PEO=0:1,　the　output　voltage　was　0.071V.　The　results　indicated　that　PLA,　PEO,　and　BT　all　exhibited　synergistic　piezoelectric　effects.　Therefore,　these　with　different　piezoelectric　properties　PLA　@　PEO　@　BT　nanofibers　were　expected　to　be　applied　in　vascular　tissue　engineering.　©　2024　SPIE.