Auto-thermal　reforming　(ATR)　is　an　effective　route　to　extract　hydrogen　from　acetic　acid　(HAc)　from　bio-oil.　Nickel-based　catalysts　were　found　active　for　conversion　of　HAc　via　the　ATR　process.　However,　the　main　concerns,　such　as　carbon　deposition　and　sintering,　led　to　deactivation　of　catalysts.　Herein,　to　address　these　issues,　a　series　of　(La,Pr)2NiO4　layered　perovskites　and　the　derived　catalysts　of　Ni/La2-2xPrxO3　were　prepared,　and　evaluated　by　ATR.　It　was　found　that　with　Pr　entering　the　La2O3　lattice,　the　reduction　temperature　of　Ni　oxide　was　decreased　with　improved　dispersion　of　Ni0;　meanwhile,　lattice　defects　were　found　within　La2-2xPr2xO3,　and　oxygen　vacancies　were　formed　with　more　reactive　oxygen　species,　promoting　gasification　of　coking　precursors.　The　lower　formation　energy　of　oxygen　vacancy　over　Pr-doped　La2O3　support　was　further　proved　by　DFT,　confirming　the　improved　oxygen　vacancies.　Therefore,　the　Ni0　&　sdot;8La1.35Pr0.73O3.92　+/-delta　catalyst　exhibited　excellent　catalytic　performance　along　with　a　stable　HAc　conversion　near　100%　and　a　hydrogen　yield　around　2.49　mol-H2/　mol-HAc,　while　no　coking　was　detected.