Quorum　sensing(QS)　is　a　crucial　aspect　of　Anammox(anaerobic　ammonium　oxidation)　technology　operation.　Given　the　difficulty　of　accumulating　Anammox　bacteria,　the　favorable　characteristics　of　sludge　are　vital　for　optimizing　the　stable　operation　of　Anammox　systems.　Therefore,　it　is　essential　to　study　the　interaction　between　QS　and　the　physical　properties　of　sludge　in　the　Anammox　process.　In　this　study,　the　extended　Derjaguin-LandauVerwey-Overbeek(XDLVO)　theory　and　rheological　properties　were　used　to　determine　the　physical　characteristics　of　different　Anammox　sludges,　subsequently　the　relationship　between　these　properties　and　signaling　molecules,　microorganisms　and　extracellular　polymeric　substances(EPS)　was　analyzed.　The　results　indicated　that　certain　physical　properties,　such　as　elasticity,　sludge　aggregation　capability,　and　hydrophobicity,　were　significantly　correlated　with　specific　signaling　molecules.　Additionally,　the　dynamic　changes　in　microbial　community　structure　were　one　of　the　main　causes　of　changes　in　the　levels　of　signaling　molecules,　which　stimulated　the　nitrogen　removal　performance　and　also　affected　the　properties　of　the　sludge.