Enzymes　are　highly　efficient　catalysts　in　nature,　governing　countless　reactions　in　biological　systems.　Enzyme-based　hydrogels　are　three-dimensional　network　materials　with　enzyme　activity,　created　by　loading　enzymes　into　hydrogels　or　using　hydrogels　to　synthesize　polymeric　materials.　Due　to　their　high　biocompatibility,　enzyme-based　hydrogels　exhibit　broad　application　potential　in　various　fields.　In　recent　years,　researchers　have　been　continuously　exploring　new　preparation　methods　to　improve　the　physical,　chemical,　and　biological　properties　of　enzyme-based　hydrogels.　Additionally,　the　introduction　of　materials　such　as　graphene,　carbon　nanotubes,　and　metal-organic　frameworks　(MOFs)　has　further　enhanced　the　biological　activity　of　these　hydrogels.　This　review　summarizes　recent　preparation　methods　and　structural　characteristics　of　enzyme-based　hydrogels,　with　a　focus　on　their　applications　in　biosensors　and　medical　fields,　highlighting　the　vast　application　prospects　of　enzyme-based　hydrogels.　©　2025　The　Author(s)